BioTechniques. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. Anal Slot Blot Hybridization. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. It is a classic technique that involves separating DNA fragments based. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. GAPDH was used as a sample loading control. ; 30 Quantification Kit. J Forensic Sci. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. RESULTS Dramatic differences with. quantification of low amounts of dsDNA. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. Southern blot analysis reveals information about DNA identity, size, and abundance. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. For. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. Dot blotting is an ideal technique for quickly. Faça loginto View Organizational & Contract. .